taraxasterol 98  (Chengdu Herbpurify CO)

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on the survival of HT-29 cells. (A) Structure of taraxasterol. (B) HT-29 cells were treated with LPS, LPS+2.5 µg/ml taraxasterol, LPS+5 µg/ml taraxasterol or LPS+10 µg/ml taraxasterol. MTT assay was performed to assess cell viability. There was no differences between control and Drugs+LPS groups. *P<0.05 vs. control; # P<0.05 vs. Drugs+LPS groups. LPS, lipopolysaccharide.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: MTT Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on secretion of IL-6 and TNF-α. HT-29 cells were treated with 10 µg/ml LPS, then with 2.5, 5 or 10 µg/ml taraxasterol. The supernatants were collected to detect the expression levels of (A) IL-6 and (B) TNF-α. # P<0.05 vs. control; *P<0.05 vs. LPS only group. TNF-α, tumor necrosis factor alpha; IL-6, interleukin 6; LPS, lipopolysaccharide.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on apoptosis rate and protein expression of BAX, cleaved caspase-3 and p53. HT-29 cells were treated with 10 µg/ml LPS, then treated with 2.5, 5 or 10 mg/ml taraxasterol. (A) Cells were analyzed by flow cytometry and (B) the apoptosis rate (%) was calculated. (C) Representative images from western blot analysis of apoptosis-related proteins and quantification of protein expression levels of (D) BAX, (E) cleaved caspase-3 and (F) p53, as a ratio to β-actin. # P<0.05 vs. control; *P<0.05 vs. LPS only group. LPS, lipopolysaccharide; BAX, bcl-2-associated X protein; p53, tumor protein p53.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Flow Cytometry, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Colonic histopathological micrographs and p53 expression levels. Mice were randomly divided into 5 groups (n=12); control group, model group (DSS group) and low, medium and high dose (25, 50 and 100 mg/kg/day respectively) taraxasterol groups. (A) Colonic tissues from each group were stained with H&E (magnification, ×100). The red arrows indicate granulation tissue hyperplasia and glandular enlargement. (B) p53 expression levels (brown staining) were measured by immunohistochemistry assay (magnification, ×100). p53, tumor protein p53; DSS, dextran sodium sulphate; H&E, hematoxylin and eosin.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Control, Staining, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on histopathological score, p53 IHC score and expression of TNF-α and IL-6 in vivo .

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, In Vivo, Control